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KMID : 0545120160260040648
Journal of Microbiology and Biotechnology
2016 Volume.26 No. 4 p.648 ~ p.658
Bacterial Species and Biochemical Characteristic Investigations of Nostoc flagelliforme Concentrates during its Storage
Yue Li Fang

Lv He Xin
Zhen Jing
Jiang Sheng Ping
Jia Shi Ru
Shen Shi Gang
Gao Lu
Dai Yu Jie
Abstract
Preservation of fresh algae plays an important role in algae seed subculture and aquaculture. The determination and examination of the changes of cell viability, composition, and bacterial species during storage would help to take suitable preservation methods to prolong the preservation time of fresh algae. Nostoc flagelliforme is a kind of edible cyanobacterium with important herbal and dietary values. This article investigated the changes of bacterial species and biochemical characteristics of fresh N. flagelliforme concentrate during natural storage. It was found that the viability of cells decreased along with the storage time. Fourteen bacteria strains in the algae concentrate were identified by PCR-DGGE and were grouped into four phyla, including Cyanobacteria, Firmicutes, Proteobacteria, and Bacteroidetes. Among them, Enterococcus viikkiensis may be a concern in the preservation. Eleven volatile organic compounds were identified from N. flagelliforme cells, in which geosmin could be treated as an indicator of the freshness of N. flagelliforme. The occurrence of indole compound may be an indicator of the degradation of cells.
KEYWORD
Nostoc flagelliforme, viability, bacteria, volatile organic compounds
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